Enzyme-based Biofuel Cell Designed for Direct Power Generation from Biofluids in Living Organisms

Enzymatic biofuel cells have attracted much attention for their potential to directly use biochemical energy sources in living organisms such as animals, fruits, etc. However, generally natural organisms have a skin, and the oxygen concentration in the organisms is lower than that of biofuels like sugars. Here, we fabricated a miniature assembly that consists of a needle bioanode for accessing biofuels in organisms through their skins and a gas-diffusion biocathode for utilizing abundant oxygen in air, for the first time. The performance of the biocathode was fourfold improved by optimizing its hydrophobicity. The assembled device with a needle anode for fructose oxidation was inserted into a raw grape, producing a maximum power of 26.5 μW (115 μW cm -2 ) at 0.34 V. A light-emitting diode (LED) with the cell served as a self-powered indicator of the sugar level in the grape. Power generation from blood sugar was also investigated by inserting a needle anode for glucose oxidation into a blood vessel in a rabbit ear. Prior coating of the tip of the needle anode with an antibiofouling agent was effective to stabilize output power.